Amperometric Determination of Serum Cholesterol with Pencil Graphite Rod

Amperometric Determination of Serum Cholesterol with Pencil Graphite Rod

A cholesterol oxidase from Streptomycin sp. was immobilized onto pencil graphite rod and employed for amperometric determination of serum cholesterol. The method has the advantage over earlier amperometric methods that it requires low potential to generate electrons from H2O2, which does not allow ionization of serum substances. The optimum working conditions of amperometric determination were ...

Electrochemical Detection of Insulin in Blood serum using Ppy/GF Nanocomposite Modified Pencil Graphite Electrode

In this study, pencil graphite electrode was modified using conductive polypyrrole (Ppy) and grapheme (GF) nanocomposite for electrochemical determination of insulin. Electrochemical behavior of insulin on PGE was investigated using cyclic voltammetric (CV) and differential pulse voltammetric (DPV) and chronoaprometry (CA) methods. Several effective parameters including pH, concentration, and s...

A simple method for the determination of serum cholesterol

Suspected Endothelial Pencil Graphite Deposition

A 14-year-old male patient had an ocular trauma with a pencil. Biomicroscopic examination revealed a broken part of pencil into the cornea. Foreign body removal and corneal wound closure were performed in the same day. After corneal repair, there was a grade 4+ anterior chamber reaction just like in preoperative examination. Dilated examination showed a very small piece broken tip of pencil on ...

Flow-Injection Amperometric Determination of Ascorbic Acid Using a Graphite-Epoxy Composite Electrode Modified with Cobalt Phthalocyanine

A flow injection method is reported for the determination of ascorbic acid based on amperometric detection, using a cobalt(II) phthalocyanine (CoPc) modified graphite-epoxy electrode. The amperometric respons was evaluated with regard to pH, ionic strength of the electrolyte, flow rate of the carrier solution, injected sample volume and conditioning time of the electrode. The limit of detec...